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Neuro Group

Group Leader Susanna Narkilahti

Publications

2024

  1. Honkamäki, L, Kulta, O, Puistola, P, Hopia, K, Emeh, P, Isosaari, L et al.. Hyaluronic Acid-Based 3D Bioprinted Hydrogel Structure for Directed Axonal Guidance and Modeling Innervation In Vitro. Adv Healthc Mater. 2024; :e2402504. doi: 10.1002/adhm.202402504. PubMed PMID:39502022 .
  2. Vinogradov, A, Kapucu, EF, Narkilahti, S. Exploring Kainic Acid-Induced Alterations in Circular Tripartite Networks with Advanced Analysis Tools. eNeuro. 2024;11 (7):. doi: 10.1523/ENEURO.0035-24.2024. PubMed PMID:39079743 PubMed Central PMC11289587.
  3. Kapucu, FE, Tujula, I, Kulta, O, Sukki, L, Ryynänen, T, Gram, H et al.. Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson’s Disease. NPJ Parkinsons Dis. 2024;10 (1):138. doi: 10.1038/s41531-024-00750-x. PubMed PMID:39069518 PubMed Central PMC11284226.

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2023
  1. Isosaari, L, Vuorenpää, H, Yrjänäinen, A, Kapucu, FE, Kelloniemi, M, Pakarinen, TK et al.. Simultaneous induction of vasculature and neuronal network formation on a chip reveals a dynamic interrelationship between cell types. Cell Commun Signal. 2023;21 (1):132. doi: 10.1186/s12964-023-01159-4. PubMed PMID:37316873 PubMed Central PMC10265920.

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2022
  1. Mörö, A, Samanta, S, Honkamäki, L, Rangasami, VK, Puistola, P, Kauppila, M et al.. Hyaluronic acid based next generation bioink for 3D bioprinting of human stem cell derived corneal stromal model with innervation. Biofabrication. 2022;15 (1):. doi: 10.1088/1758-5090/acab34. PubMed PMID:36579828 .
  2. Karttunen, J, Heiskanen, M, Joki, T, Hyysalo, A, Navarro-Ferrandis, V, Miettinen, S et al.. Effect of cell culture media on extracellular vesicle secretion from mesenchymal stromal cells and neurons. Eur J Cell Biol. 2022;101 (4):151270. doi: 10.1016/j.ejcb.2022.151270. PubMed PMID:35987046 .
  3. Räsänen, N, Harju, V, Joki, T, Narkilahti, S. Practical guide for preparation, computational reconstruction and analysis of 3D human neuronal networks in control and ischaemic conditions. Development. 2022;149 (20):. doi: 10.1242/dev.200012. PubMed PMID:35929583 PubMed Central PMC9440753.
  4. Lotila, J, Hyvärinen, T, Skottman, H, Airas, L, Narkilahti, S, Hagman, S et al.. Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient. Stem Cell Res. 2022;63 :102865. doi: 10.1016/j.scr.2022.102865. PubMed PMID:35843021 .
  5. Samanta, S, Ylä-Outinen, L, Rangasami, VK, Narkilahti, S, Oommen, OP. Corrigendum to ‘Bidirectional cell-matrix interaction dictates neuronal network formation in a brain mimetic 3D scaffold’ [Acta Biomaterialia (2022) 314-323]. Acta Biomater. 2022;147 :439. doi: 10.1016/j.actbio.2022.05.025. PubMed PMID:35597769 .
  6. Kapucu, FE, Vinogradov, A, Hyvärinen, T, Ylä-Outinen, L, Narkilahti, S. Comparative microelectrode array data of the functional development of hPSC-derived and rat neuronal networks. Sci Data. 2022;9 (1):120. doi: 10.1038/s41597-022-01242-4. PubMed PMID:35354837 PubMed Central PMC8969177.
  7. Häkli, M, Jäntti, S, Joki, T, Sukki, L, Tornberg, K, Aalto-Setälä, K et al.. Human Neurons Form Axon-Mediated Functional Connections with Human Cardiomyocytes in Compartmentalized Microfluidic Chip. Int J Mol Sci. 2022;23 (6):. doi: 10.3390/ijms23063148. PubMed PMID:35328569 PubMed Central PMC8955890.
  8. Mzezewa, R, Lotila, J, Kiiski, H, Vinogradov, A, Kapucu, FE, Peltola, J et al.. A kainic acid-induced seizure model in human pluripotent stem cell-derived cortical neurons for studying the role of IL-6 in the functional activity. Stem Cell Res. 2022;60 :102665. doi: 10.1016/j.scr.2022.102665. PubMed PMID:35091307 .
  9. Samanta, S, Ylä-Outinen, L, Rangasami, VK, Narkilahti, S, Oommen, OP. Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold. Acta Biomater. 2022;140 :314-323. doi: 10.1016/j.actbio.2021.12.010. PubMed PMID:34902615 .

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2021
  1. Samanta, S, Ylä-Outinen, L, Rangasami, VK, Narkilahti, S, Oommen, OP. Bidirectional cell-matrix interaction dictates neuronal network formation in a brain-mimetic 3D scaffold. Acta Biomater. 2022;140 :314-323. doi: 10.1016/j.actbio.2021.12.010. PubMed PMID:34902615 .
  2. Honkamäki, L, Joki, T, Grigoryev, NA, Levon, K, Ylä-Outinen, L, Narkilahti, S et al.. Novel method to produce a layered 3D scaffold for human pluripotent stem cell-derived neuronal cells. J Neurosci Methods. 2021;350 :109043. doi: 10.1016/j.jneumeth.2020.109043. PubMed PMID:33345946 .

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2020
  1. Honkamäki, L, Joki, T, Grigoryev, NA, Levon, K, Ylä-Outinen, L, Narkilahti, S et al.. Novel method to produce a layered 3D scaffold for human pluripotent stem cell-derived neuronal cells. J Neurosci Methods. 2021;350 :109043. doi: 10.1016/j.jneumeth.2020.109043. PubMed PMID:33345946 .
  2. Juntunen, M, Hagman, S, Moisan, A, Narkilahti, S, Miettinen, S. Oxygen-Glucose Deprivation-Induced Stroke Models with Human Neuroblastoma Cell- and Induced Pluripotent Stem Cell-Derived Neurons. Stem Cells Int. 2020;2020 :8841026. doi: 10.1155/2020/8841026. PubMed PMID:33178286 PubMed Central PMC7647751.
  3. Pelkonen, A, Mzezewa, R, Sukki, L, Ryynänen, T, Kreutzer, J, Hyvärinen, T et al.. A modular brain-on-a-chip for modelling epileptic seizures with functionally connected human neuronal networks. Biosens Bioelectron. 2020;168 :112553. doi: 10.1016/j.bios.2020.112553. PubMed PMID:32877779 .
  4. Välimäki, H, Hyvärinen, T, Leivo, J, Iftikhar, H, Pekkanen-Mattila, M, Rajan, DK et al.. Covalent immobilization of luminescent oxygen indicators reduces cytotoxicity. Biomed Microdevices. 2020;22 (2):41. doi: 10.1007/s10544-020-00495-3. PubMed PMID:32494857 PubMed Central PMC7270993.
  5. Ryynänen, T, Mzezewa, R, Meriläinen, E, Hyvärinen, T, Lekkala, J, Narkilahti, S et al.. Transparent Microelectrode Arrays Fabricated by Ion Beam Assisted Deposition for Neuronal Cell in Vitro Recordings. Micromachines (Basel). 2020;11 (5):. doi: 10.3390/mi11050497. PubMed PMID:32423145 PubMed Central PMC7281740.

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2019
  1. Hyvärinen, T, Hyysalo, A, Kapucu, FE, Aarnos, L, Vinogradov, A, Eglen, SJ et al.. Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures. Sci Rep. 2019;9 (1):17125. doi: 10.1038/s41598-019-53647-8. PubMed PMID:31748598 PubMed Central PMC6868015.
  2. Hyvärinen, T, Hagman, S, Ristola, M, Sukki, L, Veijula, K, Kreutzer, J et al.. Co-stimulation with IL-1β and TNF-α induces an inflammatory reactive astrocyte phenotype with neurosupportive characteristics in a human pluripotent stem cell model system. Sci Rep. 2019;9 (1):16944. doi: 10.1038/s41598-019-53414-9. PubMed PMID:31729450 PubMed Central PMC6858358.
  3. Ylä-Outinen, L, Harju, V, Joki, T, Koivisto, JT, Karvinen, J, Kellomäki, M et al.. Screening of Hydrogels for Human Pluripotent Stem Cell-Derived Neural Cells: Hyaluronan-Polyvinyl Alcohol-Collagen-Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold. Macromol Biosci. 2019;19 (7):e1900096. doi: 10.1002/mabi.201900096. PubMed PMID:31173471 .
  4. Ylä-Outinen, L, Tanskanen, JMA, Kapucu, FE, Hyysalo, A, Hyttinen, JAK, Narkilahti, S et al.. Advances in Human Stem Cell-Derived Neuronal Cell Culturing and Analysis. Adv Neurobiol. 2019;22 :299-329. doi: 10.1007/978-3-030-11135-9_13. PubMed PMID:31073942 .
  5. Aspatwar, A, Tolvanen, MEE, Schneider, HP, Becker, HM, Narkilahti, S, Parkkila, S et al.. Catalytically inactive carbonic anhydrase-related proteins enhance transport of lactate by MCT1. FEBS Open Bio. 2019;9 (7):1204-1211. doi: 10.1002/2211-5463.12647. PubMed PMID:31033227 PubMed Central PMC6609565.
  6. Ryynänen, T, Pelkonen, A, Grigoras, K, Ylivaara, OME, Hyvärinen, T, Ahopelto, J et al.. Microelectrode Array With Transparent ALD TiN Electrodes. Front Neurosci. 2019;13 :226. doi: 10.3389/fnins.2019.00226. PubMed PMID:30967754 PubMed Central PMC6438859.
  7. Hagman, S, Mäkinen, A, Ylä-Outinen, L, Huhtala, H, Elovaara, I, Narkilahti, S et al.. Effects of inflammatory cytokines IFN-γ, TNF-α and IL-6 on the viability and functionality of human pluripotent stem cell-derived neural cells. J Neuroimmunol. 2019;331 :36-45. doi: 10.1016/j.jneuroim.2018.07.010. PubMed PMID:30195439 .

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2018
  1. Ryynänen, T, Toivanen, M, Salminen, T, Ylä-Outinen, L, Narkilahti, S, Lekkala, J et al.. Ion Beam Assisted E-Beam Deposited TiN Microelectrodes-Applied to Neuronal Cell Culture Medium Evaluation. Front Neurosci. 2018;12 :882. doi: 10.3389/fnins.2018.00882. PubMed PMID:30568570 PubMed Central PMC6290344.
  2. Hagman, S, Mäkinen, A, Ylä-Outinen, L, Huhtala, H, Elovaara, I, Narkilahti, S et al.. Effects of inflammatory cytokines IFN-γ, TNF-α and IL-6 on the viability and functionality of human pluripotent stem cell-derived neural cells. J Neuroimmunol. 2019;331 :36-45. doi: 10.1016/j.jneuroim.2018.07.010. PubMed PMID:30195439 .
  3. Mäkinen, ME, Ylä-Outinen, L, Narkilahti, S. GABA and Gap Junctions in the Development of Synchronized Activity in Human Pluripotent Stem Cell-Derived Neural Networks. Front Cell Neurosci. 2018;12 :56. doi: 10.3389/fncel.2018.00056. PubMed PMID:29559893 PubMed Central PMC5845705.
  4. Paavilainen, T, Pelkonen, A, Mäkinen, ME, Peltola, M, Huhtala, H, Fayuk, D et al.. Effect of prolonged differentiation on functional maturation of human pluripotent stem cell-derived neuronal cultures. Stem Cell Res. 2018;27 :151-161. doi: 10.1016/j.scr.2018.01.018. PubMed PMID:29414606 .

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