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Biophysics of the Eye

Nymark lab

Publications

See our doctoral theses here

Articles

2025

  1. Milicevic, N, Sandu, C, Challet, E, Ihalainen, TO, Nymark, S, Felder-Schmittbuhl, MP et al.. Circadian Rhythms in Murine Ocular Tissues Including Sclera Are Affected by Neurobasal A Medium Preincubation and Mouse Strain, but Not Sex. Invest Ophthalmol Vis Sci. 2025;66 (6):62. doi: 10.1167/iovs.66.6.62. PubMed PMID:40540259 PubMed Central PMC12184798.

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2024

Azzari, L, Vippola, M, Nymark, S, Ihalainen, TO, Mäntylä, E. Iterative Immunostaining and NEDD Denoising for Improved Signal-To-Noise Ratio in ExM-LSCM. Bio Protoc. 2024;14 (18):e5072. doi: 10.21769/BioProtoc.5072. PubMed PMID:39346757. PubMed Central PMC11427331.

2023

Tervonen, A, Korpela, S, Nymark, S, Hyttinen, J, Ihalainen, TO. The Effect of Substrate Stiffness on Elastic Force Transmission in the Epithelial Monolayers over Short Timescales. Cell Mol Bioeng. 2023;16 (5–6):475–495. doi: 10.1007/s12195-023-00772-0. PubMed PMID:38099211. PubMed Central PMC10716100.

Peussa, H, Fedele, C, Tran, H, Marttinen, M, Fadjukov, J, Mäntylä, E et al.. Light-Induced Nanoscale Deformation in Azobenzene Thin Film Triggers Rapid Intracellular Ca Increase via Mechanosensitive Cation Channels. Adv Sci (Weinh). 2023;10 (35):e2206190. doi: 10.1002/advs.202206190. PubMed PMID:37946608. PubMed Central PMC10724422.

Mäntylä, E, Montonen, T, Azzari, L, Mattola, S, Hannula, M, Vihinen-Ranta, M et al.. Iterative immunostaining combined with expansion microscopy and image processing reveals nanoscopic network organization of nuclear lamina. Mol Biol Cell. 2023;34 (9):br13. doi: 10.1091/mbc.E22-09-0448. PubMed PMID:37342871. PubMed Central PMC10398900.

Karema-Jokinen, V, Koskela, A, Hytti, M, Hongisto, H, Viheriälä, T, Liukkonen, M et al.. Crosstalk of protein clearance, inflammasome, and Ca channels in retinal pigment epithelium derived from age-related macular degeneration patients. J Biol Chem. 2023;299 (6):104770. doi: 10.1016/j.jbc.2023.104770. PubMed PMID:37137441. PubMed Central PMC10240424.

Ignatova, I, Frolov, R, Nymark, S. The retinal pigment epithelium displays electrical excitability and lateral signal spreading. BMC Biol. 2023;21 (1):84. doi: 10.1186/s12915-023-01559-5. PubMed PMID:37069561. PubMed Central PMC10111697.

2022

  1. Isomäki M, Fedele C, Kääriäinen L, Mäntylä E, Nymark S, Ihalainen TO & Priimägi A. (2022) Light-Responsive Bilayer Cell Culture Platform for Reversible Cell Guidance. Small Science, 2, 2100099. https://doi.org/10.1002/smsc.202100099

  1. Peussa, H, Kreutzer, J, Mäntylä, E, Mäki, AJ, Nymark, S, Kallio, P et al.. Pneumatic equiaxial compression device for mechanical manipulation of epithelial cell packing and physiology. PLoS One. 2022;17 (6):e0268570. doi: 10.1371/journal.pone.0268570. PubMed PMID:35657824 PubMed Central PMC9165817.
  2. Korkka, I, Skottman, H, Nymark, S. Heterogeneity of Potassium Channels in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium. Stem Cells Transl Med. 2022;11 (7):753-766. doi: 10.1093/stcltm/szac029. PubMed PMID:35639962 PubMed Central PMC9299513.
  3. Fadjukov, J, Wienbar, S, Hakanen, S, Aho, V, Vihinen-Ranta, M, Ihalainen, TO et al.. Gap junctions and connexin hemichannels both contribute to the electrical properties of retinal pigment epithelium. J Gen Physiol. 2022;154 (4):. doi: 10.1085/jgp.202112916. PubMed PMID:35275193 PubMed Central PMC8922333.
  4. Viheriälä, T, Hongisto, H, Sorvari, J, Skottman, H, Nymark, S, Ilmarinen, T et al.. Cell maturation influences the ability of hESC-RPE to tolerate cellular stress. Stem Cell Res Ther. 2022;13 (1):30. doi: 10.1186/s13287-022-02712-7. PubMed PMID:35073969 PubMed Central PMC8785579.
  5. Isomäki, M, Fedele, C, Kääriäinen, L, Mäntylä, E, Nymark, S, Ihalainen, TO et al.. Light-Responsive Bilayer Cell Culture Platform for Reversible Cell Guidance. Small Sci. 2022;2 (3):2100099. doi: 10.1002/smsc.202100099. PubMed PMID:40213278 PubMed Central PMC11935986.

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2021

  1. Isomäki, M, Fedele, C, Kääriäinen, L, Mäntylä, E, Nymark, S, Ihalainen, TO et al.. Light-Responsive Bilayer Cell Culture Platform for Reversible Cell Guidance. Small Sci. 2022;2 (3):2100099. doi: 10.1002/smsc.202100099. PubMed PMID:40213278 PubMed Central PMC11935986.
  2. Viheriälä, T, Sorvari, J, Ihalainen, TO, Mörö, A, Grönroos, P, Schlie-Wolter, S et al.. Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE. Sci Rep. 2021;11 (1):933. doi: 10.1038/s41598-020-79638-8. PubMed PMID:33441679 PubMed Central PMC7806758.

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2020

  1. Fedele, C, Mäntylä, E, Belardi, B, Hamkins-Indik, T, Cavalli, S, Netti, PA et al.. Azobenzene-based sinusoidal surface topography drives focal adhesion confinement and guides collective migration of epithelial cells. Sci Rep. 2020;10 (1):15329. doi: 10.1038/s41598-020-71567-w. PubMed PMID:32948792 PubMed Central PMC7501301.

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2019

  1. Sorvari, J, Viheriälä, T, Ilmarinen, T, Ihalainen, TO, Nymark, S. Analysis of ATP-Induced Ca Responses at Single Cell Level in Retinal Pigment Epithelium Monolayers. Adv Exp Med Biol. 2019;1185 :525-530. doi: 10.1007/978-3-030-27378-1_86. PubMed PMID:31884665 .
  2. Johansson, JK, Karema-Jokinen, VI, Hakanen, S, Jylhä, A, Uusitalo, H, Vihinen-Ranta, M et al.. Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium. BMC Biol. 2019;17 (1):63. doi: 10.1186/s12915-019-0681-1. PubMed PMID:31412898 PubMed Central PMC6694495.
  3. Alarautalahti, V, Ragauskas, S, Hakkarainen, JJ, Uusitalo-Järvinen, H, Uusitalo, H, Hyttinen, J et al.. Viability of Mouse Retinal Explant Cultures Assessed by Preservation of Functionality and Morphology. Invest Ophthalmol Vis Sci. 2019;60 (6):1914-1927. doi: 10.1167/iovs.18-25156. PubMed PMID:31042799 .
  4. Tervonen, A, Ihalainen, TO, Nymark, S, Hyttinen, J. Structural dynamics of tight junctions modulate the properties of the epithelial barrier. PLoS One. 2019;14 (4):e0214876. doi: 10.1371/journal.pone.0214876. PubMed PMID:30964903 PubMed Central PMC6456171.
  5. Korkka, I, Viheriälä, T, Juuti-Uusitalo, K, Uusitalo-Järvinen, H, Skottman, H, Hyttinen, J et al.. Functional Voltage-Gated Calcium Channels Are Present in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium. Stem Cells Transl Med. 2019;8 (2):179-193. doi: 10.1002/sctm.18-0026. PubMed PMID:30394009 PubMed Central PMC6344904.

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2018

  1. Alarautalahti V, Hiltunen M, Onnela N, Nymark S, Kellomäki M & Hyttinen J. (2018) Polypyrrole-coated electrodes show thickness-dependent stability in different conditions during 42-day follow-up in vitro. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 106(6):2202-2213. https://doi.org/10.1002/jbm.b.34024

2017

  1. Korkka, I, Viheriälä, T, Juuti-Uusitalo, K, Uusitalo-Järvinen, H, Skottman, H, Hyttinen, J et al.. Functional Voltage-Gated Calcium Channels Are Present in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium. Stem Cells Transl Med. 2019;8 (2):179-193. doi: 10.1002/sctm.18-0026. PubMed PMID:30394009 PubMed Central PMC6344904.

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2017

  1. Korkka I*, Johansson JK*, Skottman H, Hyttinen J & Nymark S. (2017) Characterization of chloride channels in human embryonic stem cell derived retinal pigment epithelium. IFMBE Proceedings. 65:454-457. *Equal contributions. https://doi.org/10.1007/978-981-10-5122-7_114

2016

  1. Berry, J, Frederiksen, R, Yao, Y, Nymark, S, Chen, J, Cornwall, C et al.. Effect of Rhodopsin Phosphorylation on Dark Adaptation in Mouse Rods. J Neurosci. 2016;36 (26):6973-87. doi: 10.1523/JNEUROSCI.3544-15.2016. PubMed PMID:27358455 PubMed Central PMC4926242.
  2. Frederiksen, R, Nymark, S, Kolesnikov, AV, Berry, JD, Adler, L 4th, Koutalos, Y et al.. Rhodopsin kinase and arrestin binding control the decay of photoactivated rhodopsin and dark adaptation of mouse rods. J Gen Physiol. 2016;148 (1):1-11. doi: 10.1085/jgp.201511538. PubMed PMID:27353443 PubMed Central PMC4924931.

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2015

  1. Ilmarinen T, Hiidenmaa H, Kööbi P, Nymark S, Sorkio A, Wang J-H, Stanzel BV, Thieltges F, Alajuuma P, Oksala O, Kataja M, Uusitalo H & Skottman H. (2015). Ultrathin Polyimide Membrane as Cell Carrier for Subretinal Transplantation of Human Embryonic Stem Cell Derived Retinal Pigment Epithelium. PLoS ONE. 10(11): e0143669.https://doi.org/10.1371/journal.pone.0143669
  2. Vainio I, Abu Khamidakh A, Paci M, Skottman H, Juuti-Uusitalo K, Hyttinen J & Nymark S. (2015) Computational Model of Ca2+ Wave Propagation in Human Retinal Pigment Epithelial ARPE-19 Cells. PLoS ONE. 10(6): e0128434. https://doi.org/10.1371/journal.pone.0128434

2014

  1. Ragauskas, S, Leinonen, H, Puranen, J, Rönkkö, S, Nymark, S, Gurevicius, K et al.. Early retinal function deficit without prominent morphological changes in the R6/2 mouse model of Huntington’s disease. PLoS One. 2014;9 (12):e113317. doi: 10.1371/journal.pone.0113317. PubMed PMID:25469887 PubMed Central PMC4254453.
  2. Wang, JS, Nymark, S, Frederiksen, R, Estevez, ME, Shen, SQ, Corbo, JC et al.. Chromophore supply rate-limits mammalian photoreceptor dark adaptation. J Neurosci. 2014;34 (34):11212-21. doi: 10.1523/JNEUROSCI.1245-14.2014. PubMed PMID:25143602 PubMed Central PMC4138333.
  3. Tervonen, A, Vainio, I, Nymark, S, Hyttinen, J. Prediction of passive drug permeability across the blood-retinal barrier. Pharm Res. 2014;31 (9):2297-311. doi: 10.1007/s11095-014-1325-3. PubMed PMID:24623477 .

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2013

  1. Juuti-Uusitalo K, Delporte C, Grégoire F, Perret J, Huhtala H, Savolainen V, Nymark S, Hyttinen J, Uusitalo H, Willermain F & Skottman H. (2013) Aquaporin expression and function in human pluripotent stem cell-derived retinal pigmented epithelial cells. Investigative Ophthalmology and Visual Sciences. 54(5)3510-9. https://doi.org/10.1167/iovs.13-11800

2012

  1. Nymark S*, Frederiksen R*, Woodruff ML, Cornwall MC & Fain GL. (2012) Bleaching of mouse rods: microspectrophotometry and suction-electrode recording. Journal of Physiology. 590(10):2353-64. *Equal contributions. https://doi.org/10.1113/jphysiol.2012.228627

  1. Subrizi, A, Hiidenmaa, H, Ilmarinen, T, Nymark, S, Dubruel, P, Uusitalo, H et al.. Generation of hESC-derived retinal pigment epithelium on biopolymer coated polyimide membranes. Biomaterials. 2012;33 (32):8047-54. doi: 10.1016/j.biomaterials.2012.07.033. PubMed PMID:22892561 .

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2011

  1. Heikkinen H, Vinberg F, Nymark S & Koskelainen A. (2011) Mesopic background lights enhance dark-adapted cone ERG flash responses in the intact mouse retina: a possible role for gap junctional decoupling. Journal of Neurophysiology. 105(5):2309-18. https://doi.org/10.1152/jn.00536.2010

2010

 

2009

  1. Heikkinen H, Nymark S, Donner K & Koskelainen A. (2009). Temperature dependence of dark-adapted sensitivity and light-adaptation in photoreceptors with A1 visual pigments: a comparison of frog L-cones and rods. Vision Research. 49: 1717-28. https://doi.org/10.1016/j.visres.2009.03.024

  1. Haldin, C, Nymark, S, Aho, AC, Koskelainen, A, Donner, K. Rod phototransduction determines the trade-off of temporal integration and speed of vision in dark-adapted toads. J Neurosci. 2009;29 (18):5716-25. doi: 10.1523/JNEUROSCI.3888-08.2009. PubMed PMID:19420240 PubMed Central PMC6665239.

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2008

  1. Heikkinen H, Nymark S & Koskelainen A. (2008). Mouse cone photoresponses obtained with electroretinogram from the isolated retina. Vision Research. 48(2): 264-272. https://doi.org/10.1016/j.visres.2007.11.005

2007

 

2006

  1. Nymark, S, Haldin, C, Tenhu, H, Koskelainen, A. A new method for measuring free drug concentration: retinal tissue as a biosensor. Invest Ophthalmol Vis Sci. 2006;47 (6):2583-8. doi: 10.1167/iovs.05-1116. PubMed PMID:16723474 .

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2005

Nymark S*, Heikkinen H, Haldin C, Donner K & Koskelainen A. (2005). Light responses and light adaptation in rat retinal rods at different temperatures. Journal of Physiology. 567.3: 923-938. *Corresponding author. https://doi.org/10.1113/jphysiol.2005.090662