Publications

2022

  1. Stone, VM, Utorova, R, Butrym, M, Sioofy-Khojine, AB, Hankaniemi, MM, Ringqvist, EE et al.. Coxsackievirus B infections are common in Cystic Fibrosis and experimental evidence supports protection by vaccination. iScience. 2022;25 (10):105070. doi: 10.1016/j.isci.2022.105070. PubMed PMID:36157581 PubMed Central PMC9490033.
  2. Yrjänäinen, A, Patrikainen, MS, Azizi, L, Tolvanen, MEE, Laitaoja, M, Jänis, J et al.. Biochemical and Biophysical Characterization of Carbonic Anhydrase VI from Human Milk and Saliva. Protein J. 2022;41 (4-5):489-503. doi: 10.1007/s10930-022-10070-9. PubMed PMID:35947329 PubMed Central PMC9464147.
  3. Azizi, L, Varela, L, Turkki, P, Mykuliak, VV, Korpela, S, Ihalainen, TO et al.. Talin variant P229S compromises integrin activation and associates with multifaceted clinical symptoms. Hum Mol Genet. 2022; :. doi: 10.1093/hmg/ddac163. PubMed PMID:35861643 .
  4. Zolfaghari Emameh, R, Barker, HR, Turpeinen, H, Parkkila, S, Hytönen, VP. A reverse vaccinology approach on transmembrane carbonic anhydrases from Plasmodium species as vaccine candidates for malaria prevention. Malar J. 2022;21 (1):189. doi: 10.1186/s12936-022-04186-7. PubMed PMID:35706028 PubMed Central PMC9199335.
  5. Urbański, LJ, Bua, S, Angeli, A, Emameh, RZ, Barker, HR, Kuuslahti, M et al.. The production and biochemical characterization of α-carbonic anhydrase from Lactobacillus rhamnosus GG. Appl Microbiol Biotechnol. 2022;106 (11):4065-4074. doi: 10.1007/s00253-022-11990-3. PubMed PMID:35612631 PubMed Central PMC9200688.
  6. Kopra, K, Hassan, N, Vuorinen, E, Valtonen, S, Mahran, R, Habib, H et al.. Rapid high-throughput compatible label-free virus particle quantification method based on time-resolved luminescence. Anal Bioanal Chem. 2022;414 (15):4509-4518. doi: 10.1007/s00216-022-04104-5. PubMed PMID:35581427 PubMed Central PMC9113738.
  7. Piesanen, J, Valjakka, J, Niemelä, S, Borgenström, M, Nikkari, S, Hytönen, V et al.. Hepcidin is potential regulator for renin activity. PLoS One. 2022;17 (4):e0267343. doi: 10.1371/journal.pone.0267343. PubMed PMID:35442992 PubMed Central PMC9020709.
  8. Lindfors, L, Sioris, P, Anttalainen, A, Korelin, K, Kontunen, A, Karjalainen, M et al.. Detection of cultured breast cancer cells from human tumor-derived matrix by differential ion mobility spectrometry. Anal Chim Acta. 2022;1202 :339659. doi: 10.1016/j.aca.2022.339659. PubMed PMID:35341512 .
  9. Heinimäki, S, Lampinen, V, Tamminen, K, Hankaniemi, MM, Malm, M, Hytönen, VP et al.. Antigenicity and immunogenicity of HA2 and M2e influenza virus antigens conjugated to norovirus-like, VP1 capsid-based particles by the SpyTag/SpyCatcher technology. Virology. 2022;566 :89-97. doi: 10.1016/j.virol.2021.12.001. PubMed PMID:34894525 .
  10. Urbański, LJ, Angeli, A, Mykuliak, VV, Azizi, L, Kuuslahti, M, Hytönen, VP et al.. Biochemical and structural characterization of beta-carbonic anhydrase from the parasite Trichomonas vaginalis. J Mol Med (Berl). 2022;100 (1):115-124. doi: 10.1007/s00109-021-02148-1. PubMed PMID:34652457 PubMed Central PMC8724216.

Search PubMed

2021

  1. Heinimäki, S, Lampinen, V, Tamminen, K, Hankaniemi, MM, Malm, M, Hytönen, VP et al.. Antigenicity and immunogenicity of HA2 and M2e influenza virus antigens conjugated to norovirus-like, VP1 capsid-based particles by the SpyTag/SpyCatcher technology. Virology. 2022;566 :89-97. doi: 10.1016/j.virol.2021.12.001. PubMed PMID:34894525 .
  2. Urbański, LJ, Angeli, A, Mykuliak, VV, Azizi, L, Kuuslahti, M, Hytönen, VP et al.. Biochemical and structural characterization of beta-carbonic anhydrase from the parasite Trichomonas vaginalis. J Mol Med (Berl). 2022;100 (1):115-124. doi: 10.1007/s00109-021-02148-1. PubMed PMID:34652457 PubMed Central PMC8724216.
  3. Leppiniemi, J, Mutahir, Z, Dulebo, A, Mikkonen, P, Nuopponen, M, Turkki, P et al.. Avidin-Conjugated Nanofibrillar Cellulose Hydrogel Functionalized with Biotinylated Fibronectin and Vitronectin Promotes 3D Culture of Fibroblasts. Biomacromolecules. 2021;22 (10):4122-4137. doi: 10.1021/acs.biomac.1c00579. PubMed PMID:34542997 .
  4. Azizi, L, Turkki, P, Huynh, N, Massera, JM, Hytönen, VP. Surface Modification of Bioactive Glass Promotes Cell Attachment and Spreading. ACS Omega. 2021;6 (35):22635-22642. doi: 10.1021/acsomega.1c02669. PubMed PMID:34514235 PubMed Central PMC8427643.
  5. Stone, VM, Butrym, M, Hankaniemi, MM, Sioofy-Khojine, AB, Hytönen, VP, Hyöty, H et al.. Coxsackievirus B Vaccines Prevent Infection-Accelerated Diabetes in NOD Mice and Have No Disease-Inducing Effect. Diabetes. 2021;70 (12):2871-2878. doi: 10.2337/db21-0193. PubMed PMID:34497136 PubMed Central PMC8660981.
  6. Reshamwala, D, Shroff, S, Sheik Amamuddy, O, Laquintana, V, Denora, N, Zacheo, A et al.. Polyphenols Epigallocatechin Gallate and Resveratrol, and Polyphenol-Functionalized Nanoparticles Prevent Enterovirus Infection through Clustering and Stabilization of the Viruses. Pharmaceutics. 2021;13 (8):. doi: 10.3390/pharmaceutics13081182. PubMed PMID:34452144 PubMed Central PMC8398301.
  7. Hyunh, NB, Palma, CSD, Rahikainen, R, Mishra, A, Azizi, L, Verne, E et al.. Surface Modification of Bioresorbable Phosphate Glasses for Controlled Protein Adsorption. ACS Biomater Sci Eng. 2021;7 (9):4483-4493. doi: 10.1021/acsbiomaterials.1c00735. PubMed PMID:34382772 PubMed Central PMC8441970.
  8. Multamäki, E, Nanekar, R, Morozov, D, Lievonen, T, Golonka, D, Wahlgren, WY et al.. Comparative analysis of two paradigm bacteriophytochromes reveals opposite functionalities in two-component signaling. Nat Commun. 2021;12 (1):4394. doi: 10.1038/s41467-021-24676-7. PubMed PMID:34285211 PubMed Central PMC8292422.
  9. Laitinen, OH, Kuusela, TP, Kukkurainen, S, Nurminen, A, Sinkkonen, A, Hytönen, VP et al.. Bacterial avidins are a widely distributed protein family in Actinobacteria, Proteobacteria and Bacteroidetes. BMC Ecol Evol. 2021;21 (1):53. doi: 10.1186/s12862-021-01784-y. PubMed PMID:33836663 PubMed Central PMC8033661.
  10. Ripamonti, M, Liaudet, N, Azizi, L, Bouvard, D, Hytönen, VP, Wehrle-Haller, B et al.. Structural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions. Commun Biol. 2021;4 (1):380. doi: 10.1038/s42003-021-01886-9. PubMed PMID:33782527 PubMed Central PMC8007706.
  11. Angeli, A, Urbański, LJ, Hytönen, VP, Parkkila, S, Supuran, CT. Activation of the β-carbonic anhydrase from the protozoan pathogen Trichomonas vaginalis with amines and amino acids. J Enzyme Inhib Med Chem. 2021;36 (1):758-763. doi: 10.1080/14756366.2021.1897802. PubMed PMID:33715570 PubMed Central PMC7952076.
  12. Lampinen, V, Heinimäki, S, Laitinen, OH, Pesu, M, Hankaniemi, MM, Blazevic, V et al.. Modular vaccine platform based on the norovirus-like particle. J Nanobiotechnology. 2021;19 (1):25. doi: 10.1186/s12951-021-00772-0. PubMed PMID:33468139 PubMed Central PMC7815183.
  13. Rasheed, A, Azizi, L, Turkki, P, Janka, M, Hytönen, VP, Tuukkanen, S et al.. Extrusion-Based Bioprinting of Multilayered Nanocellulose Constructs for Cell Cultivation Using In Situ Freezing and Preprint CaCl2 Cross-Linking. ACS Omega. 2021;6 (1):569-578. doi: 10.1021/acsomega.0c05036. PubMed PMID:33458509 PubMed Central PMC7807796.
  14. Azizi, L, Cowell, AR, Mykuliak, VV, Goult, BT, Turkki, P, Hytönen, VP et al.. Cancer associated talin point mutations disorganise cell adhesion and migration. Sci Rep. 2021;11 (1):347. doi: 10.1038/s41598-020-77911-4. PubMed PMID:33431906 PubMed Central PMC7801617.
  15. Urbański, LJ, Angeli, A, Hytönen, VP, Di Fiore, A, De Simone, G, Parkkila, S et al.. Inhibition of the β-carbonic anhydrase from the protozoan pathogen Trichomonas vaginalis with sulphonamides. J Enzyme Inhib Med Chem. 2021;36 (1):329-334. doi: 10.1080/14756366.2020.1863958. PubMed PMID:33356653 PubMed Central PMC7782162.
  16. Gunnarsson, A, Stubbs, CJ, Rawlins, PB, Taylor-Newman, E, Lee, WC, Geschwindner, S et al.. Regenerable Biosensors for Small-Molecule Kinetic Characterization Using SPR. SLAS Discov. 2021;26 (5):730-739. doi: 10.1177/2472555220975358. PubMed PMID:33289457 .

Search PubMed

2020

  1. Rasheed, A, Azizi, L, Turkki, P, Janka, M, Hytönen, VP, Tuukkanen, S et al.. Extrusion-Based Bioprinting of Multilayered Nanocellulose Constructs for Cell Cultivation Using In Situ Freezing and Preprint CaCl2 Cross-Linking. ACS Omega. 2021;6 (1):569-578. doi: 10.1021/acsomega.0c05036. PubMed PMID:33458509 PubMed Central PMC7807796.
  2. Gunnarsson, A, Stubbs, CJ, Rawlins, PB, Taylor-Newman, E, Lee, WC, Geschwindner, S et al.. Regenerable Biosensors for Small-Molecule Kinetic Characterization Using SPR. SLAS Discov. 2021;26 (5):730-739. doi: 10.1177/2472555220975358. PubMed PMID:33289457 .
  3. Zhang, P, Azizi, L, Kukkurainen, S, Gao, T, Baikoghli, M, Jacquier, MC et al.. Crystal structure of the FERM-folded talin head reveals the determinants for integrin binding. Proc Natl Acad Sci U S A. 2020;117 (51):32402-32412. doi: 10.1073/pnas.2014583117. PubMed PMID:33288722 PubMed Central PMC7768682.
  4. Urbański, LJ, Angeli, A, Hytönen, VP, Di Fiore, A, Parkkila, S, De Simone, G et al.. Inhibition of the newly discovered β‑carbonic anhydrase from the protozoan pathogen Trichomonas vaginalis with inorganic anions and small molecules. J Inorg Biochem. 2020;213 :111274. doi: 10.1016/j.jinorgbio.2020.111274. PubMed PMID:33068968 .
  5. Kukkurainen, S, Azizi, L, Zhang, P, Jacquier, MC, Baikoghli, M, von Essen, M et al.. The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering. J Cell Sci. 2020;133 (19):. doi: 10.1242/jcs.239202. PubMed PMID:33046605 .
  6. Urbanski, LJ, Bua, S, Angeli, A, Kuuslahti, M, Hytönen, VP, Supuran, CT et al.. Sulphonamide inhibition profile of Staphylococcus aureus β-carbonic anhydrase. J Enzyme Inhib Med Chem. 2020;35 (1):1834-1839. doi: 10.1080/14756366.2020.1826942. PubMed PMID:32972256 PubMed Central PMC7534311.
  7. Saarinen, NVV, Lehtonen, J, Veijola, R, Lempainen, J, Knip, M, Hyöty, H et al.. Correction: Saarinen, N.V.V., et al. Multiplexed High-Throughput Serological Assay for Human Enteroviruses. Microorganismis 2020, 8, 963. Microorganisms. 2020;8 (9):. doi: 10.3390/microorganisms8091426. PubMed PMID:32957450 PubMed Central PMC7563745.
  8. Hankaniemi, MM, Baikoghli, MA, Stone, VM, Xing, L, Väätäinen, O, Soppela, S et al.. Structural Insight into CVB3-VLP Non-Adjuvanted Vaccine. Microorganisms. 2020;8 (9):. doi: 10.3390/microorganisms8091287. PubMed PMID:32846899 PubMed Central PMC7565060.
  9. Mykuliak, VV, Sikora, M, Booth, JJ, Cieplak, M, Shalashilin, DV, Hytönen, VP et al.. Mechanical Unfolding of Proteins-A Comparative Nonequilibrium Molecular Dynamics Study. Biophys J. 2020;119 (5):939-949. doi: 10.1016/j.bpj.2020.07.030. PubMed PMID:32822586 PubMed Central PMC7474207.
  10. Vasala, A, Hytönen, VP, Laitinen, OH. Modern Tools for Rapid Diagnostics of Antimicrobial Resistance. Front Cell Infect Microbiol. 2020;10 :308. doi: 10.3389/fcimb.2020.00308. PubMed PMID:32760676 PubMed Central PMC7373752.
  11. Heinimäki, S, Tamminen, K, Hytönen, VP, Malm, M, Blazevic, V. Rotavirus Inner Capsid VP6 Acts as an Adjuvant in Formulations with Particulate Antigens Only. Vaccines (Basel). 2020;8 (3):. doi: 10.3390/vaccines8030365. PubMed PMID:32645976 PubMed Central PMC7565724.
  12. Saarinen, NVV, Lehtonen, J, Veijola, R, Lempainen, J, Knip, M, Hyöty, H et al.. Multiplexed High-Throughput Serological Assay for Human Enteroviruses. Microorganisms. 2020;8 (6):. doi: 10.3390/microorganisms8060963. PubMed PMID:32604930 PubMed Central PMC7355947.
  13. Urbański, LJ, Di Fiore, A, Azizi, L, Hytönen, VP, Kuuslahti, M, Buonanno, M et al.. Biochemical and structural characterisation of a protozoan beta-carbonic anhydrase from Trichomonas vaginalis. J Enzyme Inhib Med Chem. 2020;35 (1):1292-1299. doi: 10.1080/14756366.2020.1774572. PubMed PMID:32515610 PubMed Central PMC7717681.
  14. Bachmann, M, Schäfer, M, Mykuliak, VV, Ripamonti, M, Heiser, L, Weißenbruch, K et al.. Induction of ligand promiscuity of αVβ3 integrin by mechanical force. J Cell Sci. 2020;133 (9):. doi: 10.1242/jcs.242404. PubMed PMID:32193334 .
  15. Hytönen, VP. (Strept)avidin as a template for ligands other than biotin: An overview. Methods Enzymol. 2020;633 :21-28. doi: 10.1016/bs.mie.2019.10.029. PubMed PMID:32046847 .
  16. Oberbichler, E, Wiesauer, M, Schlögl, E, Stangl, J, Faschinger, F, Knör, G et al.. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein. Methods Enzymol. 2020;633 :1-20. doi: 10.1016/bs.mie.2019.10.028. PubMed PMID:32046840 .
  17. Saarinen, NVV, Stone, VM, Hankaniemi, MM, Mazur, MA, Vuorinen, T, Flodström-Tullberg, M et al.. Antibody Responses against Enterovirus Proteases are Potential Markers for an Acute Infection. Viruses. 2020;12 (1):. doi: 10.3390/v12010078. PubMed PMID:31936473 PubMed Central PMC7020046.
  18. Baster, Z, Li, L, Kukkurainen, S, Chen, J, Pentikäinen, O, Győrffy, B et al.. Cyanidin-3-glucoside binds to talin and modulates colon cancer cell adhesions and 3D growth. FASEB J. 2020;34 (2):2227-2237. doi: 10.1096/fj.201900945R. PubMed PMID:31916632 .
  19. Chronopoulos, A, Thorpe, SD, Cortes, E, Lachowski, D, Rice, AJ, Mykuliak, VV et al.. Syndecan-4 tunes cell mechanics by activating the kindlin-integrin-RhoA pathway. Nat Mater. 2020;19 (6):669-678. doi: 10.1038/s41563-019-0567-1. PubMed PMID:31907416 PubMed Central PMC7260055.

Search PubMed

2019
  1. Hytönen, VP. (Strept)avidin as a template for ligands other than biotin: An overview. Methods Enzymol. 2020;633 :21-28. doi: 10.1016/bs.mie.2019.10.029. PubMed PMID:32046847 .
  2. Oberbichler, E, Wiesauer, M, Schlögl, E, Stangl, J, Faschinger, F, Knör, G et al.. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein. Methods Enzymol. 2020;633 :1-20. doi: 10.1016/bs.mie.2019.10.028. PubMed PMID:32046840 .
  3. Keeble, AH, Turkki, P, Stokes, S, Khairil Anuar, INA, Rahikainen, R, Hytönen, VP et al.. Approaching infinite affinity through engineering of peptide-protein interaction. Proc Natl Acad Sci U S A. 2019; :. doi: 10.1073/pnas.1909653116. PubMed PMID:31822621 PubMed Central PMC6936558.
  4. Laajala, M, Hankaniemi, MM, Määttä, JAE, Hytönen, VP, Laitinen, OH, Marjomäki, V et al.. Host Cell Calpains Can Cleave Structural Proteins from the Enterovirus Polyprotein. Viruses. 2019;11 (12):. doi: 10.3390/v11121106. PubMed PMID:31795245 PubMed Central PMC6950447.
  5. Heinimäki, S, Hankaniemi, MM, Sioofy-Khojine, AB, Laitinen, OH, Hyöty, H, Hytönen, VP et al.. Combination of three virus-derived nanoparticles as a vaccine against enteric pathogens; enterovirus, norovirus and rotavirus. Vaccine. 2019;37 (51):7509-7518. doi: 10.1016/j.vaccine.2019.09.072. PubMed PMID:31585726 .
  6. Parveen, N, Rydell, GE, Larson, G, Hytönen, VP, Zhdanov, VP, Höök, F et al.. Competition for Membrane Receptors: Norovirus Detachment via Lectin Attachment. J Am Chem Soc. 2019;141 (41):16303-16311. doi: 10.1021/jacs.9b06036. PubMed PMID:31533424 .
  7. Hankaniemi, MM, Stone, VM, Andrejeff, T, Heinimäki, S, Sioofy-Khojine, AB, Marjomäki, V et al.. Formalin treatment increases the stability and immunogenicity of coxsackievirus B1 VLP vaccine. Antiviral Res. 2019;171 :104595. doi: 10.1016/j.antiviral.2019.104595. PubMed PMID:31491431 .
  8. Hankaniemi, MM, Stone, VM, Sioofy-Khojine, AB, Heinimäki, S, Marjomäki, V, Hyöty, H et al.. A comparative study of the effect of UV and formalin inactivation on the stability and immunogenicity of a Coxsackievirus B1 vaccine. Vaccine. 2019;37 (40):5962-5971. doi: 10.1016/j.vaccine.2019.08.037. PubMed PMID:31471148 .
  9. Gering, C, Koivisto, JT, Parraga, J, Leppiniemi, J, Vuornos, K, Hytönen, VP et al.. Design of modular gellan gum hydrogel functionalized with avidin and biotinylated adhesive ligands for cell culture applications. PLoS One. 2019;14 (8):e0221931. doi: 10.1371/journal.pone.0221931. PubMed PMID:31469884 PubMed Central PMC6716642.
  10. Bachmann, M, Kukkurainen, S, Hytönen, VP, Wehrle-Haller, B. Cell Adhesion by Integrins. Physiol Rev. 2019;99 (4):1655-1699. doi: 10.1152/physrev.00036.2018. PubMed PMID:31313981 .
  11. Lemma, T, Wang, J, Arstila, K, Hytönen, VP, Toppari, JJ. Identifying yeasts using surface enhanced Raman spectroscopy. Spectrochim Acta A Mol Biomol Spectrosc. 2019;218 :299-307. doi: 10.1016/j.saa.2019.04.010. PubMed PMID:31005737 .
  12. Soto-Ribeiro, M, Kastberger, B, Bachmann, M, Azizi, L, Fouad, K, Jacquier, MC et al.. β1D integrin splice variant stabilizes integrin dynamics and reduces integrin signaling by limiting paxillin recruitment. J Cell Sci. 2019;132 (8):. doi: 10.1242/jcs.224493. PubMed PMID:30890648 .
  13. Rahikainen, R, Öhman, T, Turkki, P, Varjosalo, M, Hytönen, VP. Talin-mediated force transmission and talin rod domain unfolding independently regulate adhesion signaling. J Cell Sci. 2019;132 (7):. doi: 10.1242/jcs.226514. PubMed PMID:30837291 .
  14. Agrawal, N, Lehtonen, SI, Uusi-Mäkelä, M, Jain, P, Viitala, S, Määttä, JAE et al.. Molecular features of steroid-binding antidins and their use for assaying serum progesterone. PLoS One. 2019;14 (2):e0212339. doi: 10.1371/journal.pone.0212339. PubMed PMID:30785944 PubMed Central PMC6382169.
  15. Ojanen, MJT, Uusi-Mäkelä, MIE, Harjula, SE, Saralahti, AK, Oksanen, KE, Kähkönen, N et al.. Intelectin 3 is dispensable for resistance against a mycobacterial infection in zebrafish (Danio rerio). Sci Rep. 2019;9 (1):995. doi: 10.1038/s41598-018-37678-1. PubMed PMID:30700796 PubMed Central PMC6353920.

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