Microphysiological Systems and Organ-on-Chip Technologies

We develop enabling technologies for microphysiological systems (MPS) and organ-on-chip (OoC) applications. Developed in close collaboration with experts in biology, medicine, and materials science, these technologies support studies of human physiology, disease mechanisms, drug responses, and cell-cell interactions, and have been applied to cardiovascular, neurological, ocular, and musculoskeletal research.

Selected Videos

Oxygen Control Technologies

Oxygen Measurement Technologies

  • Luminescence lifetime imaging integrated with selective-plane illumination microscopy for oxygen imaging in 3D (Välimäki et al., 2026)
  • Oxygen imaging system for perfused 3D bone scaffolds (Välimäki et al., 2025)
  • Biocompatible immobilized luminescent oxygen sensor technology for long-term cell studies (Välimäki et al., 2020)
  • Luminescent oxygen sensor technology for in vitro cell models (Välimäki et al., 2017)

Organ-on-Chip Platforms for Selected Applications

Mechanical Cell Stimulation Technologies (flow-based, topography-based, stretching-based)

  • OoC platform for compartment-specific perfusion (Tornberg et al., 2025)
  • Equiaxial pneumatically actuated cell stretching platform (Zhao at al., 2014) for cardiac (Kreutzer et al., 2014) and osteogenic differentiation (Virjula et al., 2017)
  • Unidirectional pneumatically actuated cell stretching platform for cardiac mechanobiology studies (Kreutzer et al., 2020)
  • Equiaxial pneumatically actuated compression device for epithelial tissues (Peussa et al., 2022)
  • Durable covalent coating for PDMS-based cell stretching platforms (Leivo et al., 2017)
  • Light-controllable nanotopography platform for directional axon growth (Ristola et al., 2021)
  • Nanocellulose-based platforms for cell and tissue engineering, including (Pajorova et al. 2020) for skin applications and (Skogberg et al. 2017) for cell alignment
  • Biomimetic hydrogel platforms for studying force transmission in the retina (Korpela et al., 2025), mechanobiology in the cornea (Kauppila et al., 2023; Koivusalo et al., 2018) and RPE-endothelial cell interaction (Calejo et al., 2020)
  • Long-term hydrophilic PDMS surface treatment technology (Hemmilä et al., 2012)

Microelectrode Arrays (MEA), Electrophysiology and Bioelectronic Technologies

  • Single-cell-level cardiac MEAs (Ryynänen et al., 2018)
  • Transparent MEAs for neuronal recordings (Ryynänen et al., 2020)
  • MEAs for flexible substrates (Bakhchova et al., µTAS 2025)
  • MEAs integrated with light-controllable nanotopography platform (Ryynänen et al., Hilton Head Workshop 2024)
  • Corrosion-resistant insulation technology for long-term MEA applications (Karttu et al., 2022)
  • Graphene field-effect transistor array characterization platform (Salpavaara et al., 2026)
  • Gas-supplied chamber for long-term neuronal MEA recordings (Kreutzer et al., 2017) and structured microenvironments for enhanced neuronal activity on MEAs (Kreutzer et al., 2012)
  • PDMS tunnel structures for directed neuronal connectivity (Toivanen et al., 2017)
  • Porous SU-8 membrane interfaces for neuronal innervation studies (Salpavaara et al., 2021)

Automated and Controlled Cell Culture Technologies

  • Simulation-based control strategies for cardiomyocyte cultures (Mäki et al., 2024)
  • Feedback control of cell culture temperature using indirect measurements (Mäki et al., 2016; Mäki et al., 2018)
  • Portable multimodal live-cell imaging and sensing platform (Kattipparambil Rajan et al., 2018) and automated video-based analysis of cardiomyocyte function (Kattipparambil Rajan et al., 2020)
  • Tool for Spectral Analysis of Oriented Structures on Cellular and Subcellular Levels (Kartasalo et al., 2015)
  • Lab Automation in Cultivation of Adherent Cells (Kuncová-Kallio et al., 2006)