Our research group investigates the diversity, function, and biomedical significance of carbonic anhydrases (CAs) across multiple biological kingdoms. Our current work focuses on α-, β-, and γ-class carbonic anhydrases found in bacteria and parasites, which are key enzyme families involved in CO₂–bicarbonate balance, pH regulation, and other physiological processes. We explore how these metalloenzymes contribute to pathogen survival and virulence, providing insight into their potential as targets for novel antimicrobial and antiparasitic strategies. Our studies also address broader questions of enzyme evolution and structural diversity within CA families. Through multidisciplinary approaches, we aim to advance understanding of carbonic anhydrases in prokaryotic and parasitic systems and to support the development of innovative therapeutic and biotechnological applications.